FIG. 5.
NF-κB activation monitored with an NF-κB-responsive reporter gene. Vero cells were transfected with a luciferase construct driven by a promoter element responsive to NF-κB. Ten hours posttransfection, the cells were infected with the indicated viruses at an MOI of 1 PFU/cell. At 14 h postinfection, cells were harvested and assayed for luciferase activity. Relative light units are given as induction (n-fold). The results show the mean values and standard errors of three independent infections. The values for ΔNS2 and ΔNS1/2 were significantly different from that for wt RSV (*, P = 6.5 × 10−9 and 1.2 × 10−10, respectively) and ΔNS1 (**, P = 1.2 × 10−5 and 1.2 × 10−7, respectively).