FIG. 4.

Gag-specific CD8⁺ T-cell responses measured by intracellular cytokine staining. Splenocytes derived from mice immunized with parental ALVAC, vCP205, or Myr⁻ vCP205 were restimulated with 20 μM Gag peptide 197-205 for 6 h. The splenocytes were then stained with avidin-peroxidase complex-labeled anti-CD8 for cell surface staining and fluorescein isothiocyanate-labeled anti-IFN-γ using an intracellular staining protocol. (A) Representative dot plots from two animals in each immunization group are shown. (B) Results from all animals are shown. The horizontal lines represent the mean percentage of IFN-γ-secreting CD8⁺ T cells in each group.