Fig. 3.

Phylogenetic analysis of SSU-rRNA sequence data (aligned over 2035 positions) to infer the relationships of recognised Blastocystis subtypes (STs) as well as new STs discovered in the present study. The tree was constructed using Bayesian Inference method (MrBayes) and used Proteromonas lacertae as an outgroup. Posterior probabilities less than 0.95% are not displayed. The two novel subtypes and additional ST13 and ST24 sequences are indicated in bold. After the present analysis was completed, Santín et al. (2023) reported a subdivision of “ST10” into four STs (i.e. ST10, ST42, ST43 and ST44).