FIG. 5.

Rh184 delays intracellular transport of MHC I molecules. HeLa cells were transiently transfected with the constructs shown and pulse-chase labeled as indicated. MHC I heterodimers were immunoprecipitated with W6/32, and the maturation state of N-linked glycosylation was determined by digestion with EndoH. (A) Retention of MHC I molecules in an EndoH-sensitive state by Rh184 and Rh185. (B) Rh186 and Rh187 do not interfere with acquisition of EndoH resistance by MHC I. (C) Pulse-labeled Rh184-transfected HeLa cells chased for a longer time. MHC I molecules become EndoH resistant after 6 h of chase. The asterisks indicate a nonspecific protein band. E^r, EndoH resistant; E^s, EndoH sensitive. The two E^s bands in panel C indicate incomplete digestion by EndoH.