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. 2023 Dec 11;32(1):101171. doi: 10.1016/j.omtm.2023.101171

Figure 1.

Figure 1

T cell selection using positive vs. negative selection

(A) Schematic of selection methods compared in this study; positive selection (anti-CD4 and anti-CD8 immunomagnetic beads) and negative selection (immunomagnetic beads targeting non-T cell subsets). Image created with biorender.com. (B) Different parameters were used to evaluate T cells obtained by positive and negative selection: (i) Purity of CD3+ cells; (ii) recovery of CD3+ cells calculated as the number of CD3+ T cells obtained after selection normalized to input CD3+ cells; and (iii) viability, measured via propidium iodide and acridine orange (AO/PI) staining immediately after selection. The B-ALL patient sample is indicated by an x. (C and D) Flow cytometry was performed on individual samples before and after selection using anti-CD19, anti-CD14/CD16, anti-CD3, and anti-CD56 monoclonal antibodies (mAbs), to distinguish B, monocyte, T, and natural killer cells, respectively. Quantifications are presented for each donor (C) as well as for each subset (D). The antibody concentrations used for negative selection were increased by 2-fold for healthy donor (HD) 4, HD5, and the patient (Pt) as compared with HD1, HD2, and HD3. ∗p < 0.05 using paired t test.