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Analysis of purified recombinant polypeptides Δ60VP1, VP1-F1, and VP1-F2, utilized in this study. The purified recombinant F1, F2, and Δ60VP1 proteins were subjected to analysis by SDS-PAGE (A) and Western blotting using His Tag Antibody as the primary antibody (B). The gel lanes are labeled as follows: Lane M: CozyHi Prestained Protein Ladder (highQu, Germany); Lane 1: Recombinant VP1-F1 (17 kDa); Lane 2: VP1-F2 (16 kDa); Lane 3: Δ60VP1 (45 kDa).
