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Specificity of monoclonal antibodies to purified recombinant polypeptides VP1-F1, VP1-F2, and Δ60VP1. The specificity of monoclonal antibodies (MABs) against purified recombinant polypeptides VP1-F1, VP1-F2, and Δ60VP1 was confirmed through Western blot analysis using primary antibodies as MAB1 (A), and MAB3 (B). Lane M: CozyHi Prestained Protein Ladder (highQu, Germany); Lane 1: Recombinant VP1-F1 (17 kDa); Lane 2: VP1-F2 (16 kDa); Lane 3: Δ60VP1 (45 kDa).
