Fig. 5.

Oxidative stress, ER stress, lipid peroxidation, and DNA damage in livers of 4 and 8 weeks old OGT^LKOmice.

Male liver-specific Ogt knockout mice (OGT^LKO) and control floxed littermates (OGT^LWT) were studied 4 and 8 weeks after birth in the fed state. (A) Relative expression levels of oxidative stress markers normalised to TBP. (B) Relative expression of Chop normalised to TBP. (C) Western blots showing protein content in whole liver lysates of markers of oxidative stress (p-p62), ER stress (CHOP), and DNA damage (γH2AX) normalised to actin. Cropped membranes are indicated with discontinued lines. (D) Quantification of p-p62, CHOP, and γH2AX is shown. (E) MDA staining in liver sections from 4- and 8-week-old OGT^LWT and OGT^LKO mice. Scale bars = 100 μm. (F) Liver sections from OGT^LWT and OGT^LKO mice at 12 weeks and 12 months of age stained with Sirius Red. Scale bars = 100 μm. Data are shown as mean ± SEM of 8–10 mice at 4 weeks, 13–15 mice at 8 weeks, 18–20 mice at 12 weeks, and 18–20 mice at 1 year. ∗p <0.05, ∗∗p <0.01, ∗∗∗p <0.001 by two-way Anova followed by Bonferroni post hoc test_. CHOP, CCAAT–enhancer-binding protein homologous protein; ER, endoplasmic reticulum; Gsta1, glutathione S-transferase alpha 1; Gstm1, glutathione S-transferase mu 1; Gstm3, glutathione S-transferase mu 3; H2AX, H2AX variant histone; MDA, malondialdehyde; NqO1, NAD(P)H quinone dehydrogenase 1; OGT, O-GlcNAc transferase; TBP, TATA-box binding protein.