FIG. 9.

pH-independent HIV transmission by iDCs. (A) Infection of iDCs by HIV(VSV) pseudotypes is inhibited by bafilomycin A1. iDCs were pretreated or not pretreated with bafilomycin A1 (Baf A1, 250 nM) for 1 h, and then pulsed with HIV-Luc(VSV) (20 ng of p24/10⁶ cells). Luciferase activity was measured 2 days later in cell lysates. (B) Bafilomycin A1 does not affect HIV transmission from iDCs to Jurkat cells. iDCs (5 × 10⁵ cells) were pretreated or not pretreated for 1 h with bafilomycin A1 or mock treated, exposed to 0.1 (left) or 0.01 (right) ng of NL4./ml for 2 h, washed, and cocultivated with Jurkat cells (5 × 10⁵ cells). As a control, Jurkat cells were exposed to the same virus inputs and cultured alone. Viral replication was monitored by measuring p24 production in culture supernatants. One of three independent experiments is shown.