Table 2.
Effects of PRP on Cell Behavior in vitro
| Author (Year) | Cell Type(s) Used | Control Group(s) | PRP Effect on Cells |
|---|---|---|---|
| Kinoshita et al (2020)31 | Human osteoblasts | Cell media only | Fresh and freeze-dried PRP increased osteoblast proliferation |
| Kanno et al (2004)129 | Human osteosarcoma cell lines HOS and SaOS-2 | Cell media only | PRP increases HOS and SaOS-2 proliferation in dose-dependent manner |
| Fernandez-Medina et al (2019)72 | Human osteoblasts | Thrombus (clot) | Reduced cell viability and migration above concentrations of 60% |
| Ferreira et al (2005)142 | Human osteoblasts | None | PRP increases osteoblast proliferation in dose-dependent manner up to 50% concentration |
| Steller et al (2019)143 | Osteoblasts and oral fibroblasts | Cell media only | PRP and PRF increased proliferation and migration of osteoblasts and fibroblasts, counteracting the negative effects of zoledronic acid |
| Ogino et al (2016)144 | Human osteosarcoma cell line SaOS-2 | Platelet poor plasma (PPP) | PRP increases cell proliferation in dose-dependent manner |
| Vahabi et al (2019)45 | MG-63 osteoblast-like cells and human fibroblasts | Cell media only | PRP increases viability and adhesion of osteoblast like cells and fibroblasts |
| Celotti et al (2015)146 | Human osteosarcoma cell line SaOS-2 | Cell media only | PRP increases cell proliferation and migration in dose-dependent manner |
| Wang et al (2018)147 | Human osteoblasts | Cell media only | iPRF more so than PRP, promoted osteoblast proliferation and migration |
| Graziani et al (2006)61 | Human osteoblasts and fibroblasts | Cell media only | PRP increases cell proliferation of both osteoblasts and fibroblasts in dose dependent manner up to 50% concentration above which caused reduced cell proliferation |
| Vahabi et al (2017)130 | MG-63 osteoblast-like cells and human fibroblasts | Cell media only | PRP did not show significant increase in cell proliferation |
| Casati et al (2015)30 | Human osteosarcoma cell line SaOS-2 | Cell media only | PRP stimulates cell migration |
| Slapnicka et al (2008)131 | Human osteoblasts | Cell media only | PRP did not significantly increase cell proliferation |
| Martinotti et al (2014)132 | Human osteosarcoma cell line SaOS-2 | Cell media only | PRP promotes cell migration and induces a mixed osteoclastic/osteogenic gene expression |
| Gaßling et al (2009)133 | Human osteosarcoma cell line SaOS-2, human osteoblasts, and human fibroblasts | Cell media only | PRP led to increased growth factor secretion compared to PRF |
| Herrera et al (2012)134 | Human osteosarcoma cell line SaOS-2 | Cell media only | PRP increases osteoblast activity and cytokine release |
| He et al (2009)135 | Rat osteoblasts | Cell media only | PRF led to gradual and sustained release of cytokines compared to PRP |
| Mazzocca et al (2012)136 | Human osteocytes, myocytes, and tenocytes | Cell media only | All forms of PRP increased cell proliferation of all cell types |
| Mooren et al (2010)137 | Rat osteoblast-like cells and human umbilical vein endothelial cells (HUVECs) | Cell media only | PRP promotes proliferation of osteoblast-like cells and promotes tubular formation in endothelial cells in a dose-dependent manner |
| Garcia-Martinez et al (2012)138 | Human osteoblasts | Cell media only | PRP increases cell proliferation and altered expression of cell-surface markers |
| Zou et al (2014)140 | Rabbit bone marrow mesenchymal stem cells (BMSCs) | Cell media only | PRP can promote proliferation and osteogenic differentiation of BMSCs |
| Bi et al (2010)139 | Goat marrow stromal cells (MSCs) | Cell media only | Cell growth and alkaline phosphatase activity greater on the TCP+PRP composite compared to TCP and cell media alone control. |
| Chen et al (2013)67 | Rate Bone marrow mesenchymal stem cells (BmMSCs) | Cell media only | Greater cell proliferation in high and medium concentration PRP. Higher alkaline phosphatase activity in low and medium concentration PRP, but inhibited activity in high concentration PRP. |
| Qi et al (2015)141 | Bone marrow mesenchymal stem cells (BmMSCs) | Cell media only | Increased expression of collagen I, collagen III, tenomodulin, and osteocalcin genes, increased alizarin red staining, and increased alkaline phosphatase activity in PRP group suggestive of ability of PRP to promote osteogenic differentiation |