FIG. 2.

Recombinant influenza virus polymerase interacts with Pol II. Lysates from 293T cells expressing the three subunits of the influenza virus polymerase or the PB2 and PA subunits as well as PB1-TAP were purified on IgG Sepharose. (A to C) Total cell lysates (top panels) and purified samples (bottom panels) were analyzed by Western blotting with monoclonal anti-Pol II CTD antibody 4H8 (A), monoclonal anti-phosphoserine 2 Pol II antibody H5 (B), and monoclonal anti-phosphoserine 5 Pol II antibody H14 (C). (D) Purified material was analyzed on an 8% polyacrylamide gel, and proteins were detected by silver staining.