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. 2024 Jan 30;14:2482. doi: 10.1038/s41598-024-52620-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MIL and its NMIL within the blood vessel system. (A) Control embryo with characteristic blood vessel system. MIL (A) 1 × and (C) 2x, respectively, where the blood vessel system is interrupted. NMIL (D) 1X and (E) 2X, respectively, in contrast to MIL, which demonstrated a minor decrease in vascular mass. Vascular densities following treatments of MIL and its NMIL. A significant reduction in vascular density can be seen in both groups that received MIL and its NMIL. The embryos that received the MIL treatment revealed less vascular mass than NMIL.