TABLE 1.

Drug susceptibility profile^a of selected BVDU^r clones and comparative neurovirulence in NMRI mice

Clone or wild type	Mutation	n-Fold increase in IC50b										Neurovirulenced log (PFU/LD50)
		BVDU	BVaraU	ACV	GCV	PCV	PFA	PMEA	PMEDAP	HPMPC	HPMPA
CI-1	Ala 168 to Thr	≥2,420	>3,450	0.9	1.0	0.92	1.3	1.5	1.7	0.7	1.3	0.05
CI-5	Ala 168 to Thr	≥2,260	≥3,450	1.0	0.9	0.73	1.2	1.0	1.1	0.8	0.9	0.03
CI-24	Arg 51 to Trp	565	>3,450	1,000	3,400	500	0.9	1.1	1.2	1.2	1.0	2.92
CI-28	Arg 51 to Trp	500	>3,450	984	10,670	450	1.1	1.5	1.8	1.4	1.6	2.92
CI-3	Gly 59 to Trp	≥2,550	>3,450	605	1,400	73	0.6	0.7	0.6	2.0	0.9	2.54
CI-27	Gly 206 to Arg	≥3,230	>3,450	≥979	20,670	>769	1.2	1.5	2.2	1.1	1.6	3.30
CI-2	Arg 220 to His	>3,230	>3,450	147	57	5.4	0.7	1.2	1.0	0.7	1.1	0.94
CI-22	Tyr 239 to Ser	113	931	247	180	20	1.0	1.5	1.5	0.8	1.1	NDe
CI-23	Tyr 239 to Ser	23	1,400	126	63	24	1.1	1.2	1.4	1.0	1.9	1.40
CI-29	Gln 261 to stop	≥3,230	>3,450	468	667	≥769	1.5	1.5	2.5	1.8	2.5	3.68
CI-25	Arg 281 to stop	≥1,770	>3,450	316	333	≥438	1.1	1.2	1.3	0.9	1.0	4.22
CI-34	Thr 287 to Met	>3,230	>3,450	≥1,050	≥33,330	>769	1.0	0.8	1.5	1.3	2.3	2.07
CI-4	146 frameshift	≥2,020	>3,450	789	4,600	>769	0.5	1.1	0.9	1.1	1.7	3.43
CI-15	185 frameshift	≥3,230	>3,450	≥947	6,270	≥627	1.1	1.5	1.0	1.3	0.7	2.5
CI-30	185 frameshift	>3,230	ND	>1,050	14,000	≥769	1.3	1.5	1.6	0.9	1.1	ND
Wild type		0.0062c	0.0058	0.019	0.00015	0.026	19.6	7.4	1.91	0.114	0.035	0.05

^aDrug susceptibility profiles of BVDU^r HSV-1 clones were determined in HEL cells.

^bThe increase in IC₅₀s was calculated from at least two independent experiments.

^cMean IC₅₀s were from seven independent experiments.

^dNeurovirulence of the different mutants was evaluated by intracerebral inoculation of the viruses into mice. The log (PFU/LD₅₀) was determined as a reciprocal parameter of neurovirulence.

^eND, not determined.