TABLE 2.
Antiviral susceptibilities of BVDUr clones in Ost TK− cells versus Ost TK−/HSV-1TK+ cells
| Clone or wild type | Mutation | IC50 (μg/ml)a
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Ost TK− cells
|
Ost TK−/HSV-1TK+ cells
|
||||||||
| ACV | GCV | PCV | PFA | ACV | GCV | PCV | PFA | ||
| CI-24 | R51W | ≥12.5 | 20 | 20 | 11.3 | 0.035 | 0.0013 | 0.035 | 20 |
| CI-28 | R51W | >20 | >20 | >20 | 14.3 | 0.029 | 0.0015 | 0.032 | 20 |
| CI-3 | G59W | ≥15 | ≥20 | ≥15 | 16 | 0.026 | 0.0026 | 0.042 | 25.1 |
| CI-27 | G206R | ≥15 | ≥20 | ≥20 | 22.6 | 0.045 | 0.0014 | 0.07 | 18 |
| CI-2 | R220H | ≥18.6 | 14.7 | 8.2 | 27.3 | 0.034 | 0.002 | 0.037 | 19 |
| CI-22 | Y239S | 5.7 | 0.95 | 7 | 19.6 | 0.043 | 0.004 | 0.07 | 25.1 |
| CI-23 | Y239S | 4.7 | 0.7 | 12 | 22.9 | 0.051 | 0.009 | 0.07 | 25.1 |
| CI-29 | Q261 stop | ≥20 | ≥15 | >20 | 12.1 | 0.036 | 0.0051 | 0.096 | 22.6 |
| CI-25 | R281 stop | 20.0 | >20 | >20 | 20.8 | 0.036 | 0.0033 | 0.06 | 25.1 |
| CI-34 | T287M | ≥20 | ≥15 | >20 | 16.3 | 0.02 | 0.002 | 0.032 | 20 |
| CI-4 | 146 frameshift | >20 | 16 | >20 | 15 | 0.033 | 0.0032 | 0.032 | 15 |
| CI-15 | 185 frameshift | >20 | >20 | >20 | 15 | 0.02 | 0.005 | 0.02 | 20 |
| CI-30 | 185 frameshift | 5 | ≥15 | ≥20 | 11.3 | 0.02 | 0.0032 | 0.032 | 20 |
| Wild type (KOS) | 0.07 | 0.0094 | 0.12 | 15 | 0.04 | 0.0034 | 0.065 | 26 | |
a
The results are mean values of two independent experiments.