TABLE 1.
Sequences of the oligonucleotides used in this study
| Designation | Sequence (5′ to 3′)a |
|---|---|
| S131R-LFF... | ACTAGTTCTAGAGCGGCCATTACGTACCCTCTAGAACCCATAGG |
| S131R-LFR2 | CGAAGAACGTTAATTAAAGTACGCTACATTATAGATAACTAAACGATAAATAAGCG |
| S131R-RFF2 | TAGCGTACTTTAATTAACGTTCTTCGGGAGTTGCTTAAATAATTTTGCTAAAATATATAAG |
| S131R-RFR | AGCTCACCGCGGTGGCGGCCCTACTCTACTGTTGCTTTGTCGTT |
| C/N1F | ACTAGTTCTAGAGCGGCCGGATCCTGCGTATATTATACACGGCC |
| C1R | TCCTCGCCCTTGCTCACCATGGCGCCAGCAACTCCTATAATCCCCCAG |
| C2F | CTGGGGGATTATAGGAGTTGCTGGCGCCATGGTGAGCAAGGGCGAGGA |
| C4F | GCATGGACGAGCTGTACAAGTAACTAAACGGGTCTTGAGGGGT |
| C5R | ACCCCTCAAGACCCGTTTAGTTACTTGTACAGCTCGTCCATGC |
| C4R | ATACATTTTAGCGAGGTTATGATGGCATTTCTTCTGAGCA |
| C6F | TGCTCAGAAGAAATGCCATCATAACCTCGCTAAAATCTAT |
| C/N3R | GCTCCACCGCGGTGGCGGCCTCTAGAGGGGGAATCTACTCTACTGTT |
a
Underlined portions are sequences homologous to the vector and common to the PCR fragments.