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. 2024 Jan 17;10:1274482. doi: 10.3389/fmed.2023.1274482

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Copyright © 2024 Stüve, Nerb, Harrer, Wuttke, Feuerer, Junger, Eggenhofer, Lungu, Laslau and Ritter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of automated organoid detection applications. (A) The total number of organoids determined by the StrataQuest Organoid App (SQ; orange lines; plus, EGF solid, w/o EGF dotted) and Incucyte^® (blue lines; plus EGF solid, w/o EGF dotted) is shown. The median from quadruplicates of n = 3 different donors plus the 95% confidence interval is depicted. (B) The Šídák’s multiple comparisons test highlights the statistical data of selected samples. (C) Representative analysis (day 5 w/o EGF) are shown. Left: StrataQuest-supported Organoid App (black arrows highlight correct exclusion of organoid- mimicking contours). Middle: Incucyte^® analysis (red arrows highlight wrong detection of organoid-mimicking contours). Right: Inserts with higher amplification are depicted. The bars represent 500 μm.