Table 2.
Key resources.
| Biological samples: recombinant proteins | ||
|---|---|---|
| Reagent or resource | Source | Identifier |
| human R-Spondin 1 | Peprotech | Cat. No.: 120-38 |
| human EGF | Peprotech | Cat. No.: AF-1000 |
| human HFG | Peprotech | Cat. No.: 100-39 |
| human FGF-10 | Peprotech | Cat. No.: 100-26 |
| human EGF-10 | Peprotech | Cat. No.: AF-1000 |
| IL-2 | Novartis | Proleukin® S |
| Software and Algorithms | ||
| OrganoSeg | Borten et al., | https://github.com/JanesLab/OrganoSeg |
| StrataQuest v7.1.1.138 |
TissueGnostics | https://tissuegnostics.com/products/contextual-image-analysis/strataquest |
| Incucyte® Organoid Analysis ModuleIncucyte | Sartorius | https://www.sartorius.com/en/products/live-cell-imaging-analysis/live-cell-analysis-software/incucyte-organoid-analysis-software |
| TissueFAXS-plates software v7.1.6245.120 | TissueGnostics | https://tissuegnostics.com/products/scanning-and-viewing-software/tissuefaxs-imaging-software |
| GraphPadPrism 9.5.1 for macOS | GraphPad Software, LLC. | https://www.graphpad.com |
| Plastic material | ||
| 48-well plate | Corning/Merck KgaA | Cat. No.: CLS3548 |
| CellStrainer | Miltenyi | Cat. No.: 130-098-462 |
| Critical cell culture components | ||
| Matrigel® | Corning | Cat. No.: 356230 |
| HEPES | PAN-Biotech | Cat. No.: P05-01100 |
| L-Glutamin | PAN-Biotech | Cat. No.: P04-80100 |
| Antibiotic-antimycotic | Gibco | Cat. No.: 15240062 |
| N2 serum-free supplement | Gibco | Cat. No.: 17502-048 |
| B-27 serum-free supplement | Gibco | Cat. No.: 12587-010 |
| N-Acetyl L-Cystein | Sigma/Merck KgaA | Cat. No.: A9165 |
| Gastrin I | Sigma/Merck KgaA | Cat. No.: G9145 |
| Nicotinamin | Sigma/Merck KgaA | Cat. No.: N0636 |
| A83-01 | BioGems/ Hölzel Diagnostika GmbH |
Cat. No.: 9094360 |
| Forskolin | R&D/Bio-Techne | Cat. No.: 1099 |
| Y-27632 | BioGems/ Hölzel Diagnostika GmbH |
Cat. No.: 1293823 |
| DPBS | Gibco | Cat. No.: 14190-094 |
| TexMACS™ Medium | Miltenyi Biotec | Cat. No.: 130-097-196 |
| T Cell TransAct™ | Miltenyi Biotec | Cat. No.: 130-111-160 |
| ADV/DMEM-F12 | Gibco | Cat. No.: 12634010 |
| EBSS | Gibco/Thermo Fisher Scientific Inc., | Cat. No.: 24010043 |
| Collagenase | SIGMA/Merck KgaA | Cat. No.: C9891 |
| Cell separation reagents | ||
| Pancoll® | PAN-Biotech | Cat. No.: P04-601000 |
| biotinylated anti-human CD8 (clone HIT8a) | BioLegend | Cat. No.: 300904 |
| anti-biotin ultrapure microbeads | Miltenyi Biotec | Cat. No.: 130-105-637 |
| Imaging hardware | ||
| TissueFAXSiPlus | TissueGnostics | https://tissuegnostics.com/products/fluorescence-brightfield-cytometer/tissuefaxs-i-plus |
| Incucyte® SX5 Live-Cell Analysis System | Sartorius | https://www.sartorius.com/en/products/live-cell-imaging-analysis/live-cell-analysis-instruments/sx5-live-cell-analysis-instrument?&utm_source=google&utm_medium=cpc&utm_campaign=incucyte&utm_term=brand&utm_content=search&gad=1&gclid=CjwKCAjw-7OlBhB8EiwAnoOEk3ycK0ZXXZanqBUwI_8aUyKfrOQNgP3NbvxddlEF7IIGWCaaQMbVhRoC0TsQAvD_BwE |