TABLE 2.
MICs of aminoglycosides against Francisella tularensis.
| References | F. tularensis strains | Method/medium | Gentamicin MICs (mg/L) | Streptomycin MICs (mg/L) | ||||
| Country source | Type (number) | Isolated in | Range | MIC90 | Range | MIC90 | ||
| Origgi et al., 2014 | Switzerland | B6 (19) | 1996–2013 | BMD / caMHB + 2% IsoVx | ≤0.12–0.25 | 0.25 | 4 | 4 |
| B12 (5) | ≤0.12–0.25 | 4 | ||||||
| Georgi et al., 2012 | Europe | B (69) | NA | BMD / caMHB + 2% IsoVx | ≤0.25–0.5 | 0.5 | ≤0.5–2 | |
| North America | A (7) | ≤0.25–0.5 | ≤2 | |||||
| Urich and Petersen, 2008 | North America | A (92) | 1974–2005 | BMD / caMHB + 2% IsoVx | 0.03–0.5 | 0.25 | 0.25–4 | 2 |
| B (77) | 0.03–0.5 | 0.12 | 0.25–2 | 2 | ||||
| Heine et al., 2017 | Variable | A (19), B (10) |
1920–2002 | BMD / caMHB + 2% IsoVx | ≤0.03–1 | 0.5 | ||
| Caspar et al., 2018 | France | B6 (59) | 2006–2017 | BMD / caMHB + 2% IsoVx | ≤0.03–0.25 | 0.125 | ||
| All BMD tests | ≤0.03–1 | 0.12–0.5 | 0.25–4 | 2–4 | ||||
| Johansson et al., 2000 | Sweden | B (7) | 1998 | E-test / mThayer-Martin | 0.5–1 | |||
| Hotta et al., 2013 | Japan | B (36) | 1926–1989 | E-test/ Chocolate II agar | 0.023–0.5 | 0.125 | ||
| Kreizinger et al., 2013 | Hungary | B12 (29) | 2003–2010 | E-test / mFrancis agar | 0.38–1 | 0.75 | 3–8 | 6 |
| Yesilyurt et al., 2011 | Turkey | B bvII (39) | 2009–2010 | E-test / GCBA | 0.094–0.25 | 0.25 | 0.75–1.5 | 1.5 |
| Tomaso et al., 2005 | Austria | B bvII (50) | 1992–1998 | E-test / CHAB | 0.094–2 | 0.75 | 0.75–8 | 3 |
| Ikäheimo et al., 2000 | Finland | B (38) | NA | E-test / CHAHb | 0.38–1.5 | 1 | 0.25–4 | 4 |
| Kiliç et al., 2013 | Turkey | B bvII (249) | 2009–2012 | E-test / CHA | 0.094–0.38 | 0.25 | 0.5–2 | 1.5 |
| Johansson et al., 2002 | USA | A (8) | 1996–2001 | E-test / MHI + 1% IsoVx or CHAB | 0.032–0.25 | 0.064–2 | ||
| B (16) | 0.016–0.125 | 0.064 | 0.064–1 | 0.25 | ||||
| Velinov et al., 2011 | Bulgaria | B bvII (21) | NA | E-test / caMHB + 2% IsoVx | 0.064–0.5 | 0.125 | 0.25–2 | 1 |
| Tomaso et al., 2017 | Germany | 1B4, 3B6, 34B12 (128) | 2005–2014 | E-test / CHAB | 0.047–2 | 1 | 0.75–8 | 4 |
| All E-tests | 0.016–2 | 0.064–1 | 0.064–8 | 0.25–6 | ||||
Methods: BMD: broth microdilution technique; E-test: E-test strip method. Culture media: caMHB: cation-adjusted MHB; mThayer-Martin : modified Thayer-Martin (i.e., Thayer-Martin agar with hemoglobin and polyvitamins supplement); mFrancis agar: modified Francis agar (i.e., chocolate agar plate containing 1% glucose and 0.1% cysteine); GCBA: glucose/cysteine blood agar (i.e., brain heart infusion agar supplemented with 1% glucose monohydrate, 0.1% L-cysteine, and 9% chocolatized sheep blood); CHA: cysteine heart agar; CHAB: CHA + 10% sheep blood; CHAHb: CHA + 2% hemoglobin; IsoVx: IsoVitaleX™.