FIG. 3.

SDS-PAGE and Western blot analysis of M. gallisepticum cultured in the presence of a pMGA-specific MAb. (a) Coomassie blue-stained molecular weight standards (lane 1) and cells grown in broth medium alone (lane 2) or grown in broth containing MAb 66 (lane 3). (b) Rabbit anti-pMGA serum was used to immunostain a Western transfer of cellular proteins from mycoplasma cells grown in broth alone (lane 1) or in broth containing MAb 66 (lane 2). Arrowheads indicate the location of the pMGA protein. Arrows indicate the positions of 45- and 82-kDa bands referred to in the text. Molecular weight protein standards (Bio-Rad) are phosphorylase b (97,400), BSA (66,200), ovalbumin (45,000), and carbonic anhydrase (31,000).