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The dotL gene can be deleted in a strain lacking dotA. Southern blot analysis of ΔdotL recombinants in a ΔdotA background. A 700-bp SalI fragment from pJB1001 encoding DNA flanking dotL on the chromosome was used as a probe to determine the status of dotL in these strains. Lane 1 is the wild-type strain Lp02; lane 2 is JV1005, a ΔdotL/dotL merodiploid in a dotA mutant background; and lanes 3 to 16 are JV1005 resolved on CYET plus 5% sucrose.
