FIG. 3.

Zymogen profiles with variations in strains, media, and temperature of growth. Lane 1, B. cereus T grown in G-tris medium at 30°C; lane 2, B. cereus T grown in NSM at 30°C; lane 3, B. cereus T grown at 37°C in G-tris; lane 4, B. cereus T grown at 37°C in NSM; lane 5, Sterne strain grown at 30°C in NSM; lane 6, Sterne strain grown at 30°C in BHI plus 0.5 M sorbitol; lane 7, Sterne strain grown at 37°C in NSM; lane 8, Sterne strain grown at 37°C in BHI plus 0.5 M sorbitol; lanes 9 and 10, Sterne strain grown at 37°C in NSM and samples incubated at 27°C for 30 min prior to loading (lane 9 supernatant from cells sampled 90 min earlier than the samples in the other lanes). Supernatants in lanes 1 to 8 and 10 are from samples taken 3 h after the end of growth (20 to 30% phase-dull spores in each). See legend to Fig. 1 for the amounts loaded.