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A clone of the cot43 gene regulates the intensity of the protease bands. Supernatants of strain St-13 (lanes 1, 4, and 7), strain St-13/cot43 (lanes 2, 5, and 8), and strain St-13/cot43P (lanes 3, 6, and 9) were used. Samples were removed during late exponential growth (lanes 1 to 3), at the end of growth (lanes 4 to 6), and 90 min later (lanes 7 to 9). Amounts were loaded as described in the legend to Fig. 1.
