TABLE 3.
Yields of Edman degradation of the untreated hexameric SH
| Cycle | HoxF
|
HoxH
|
HoxU
|
HoxY
|
HoxI
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| aaa | pmolb | Relative amtc | aa | pmol | Relative amt | aa | pmol | Relative amt | aa | pmol | Relative amt | aa | pmol | Relative amt | |
| 3 | S | 2.29 | 1 | K | 3.01 | 1.31 | Q | 3.9 | 1.70 | A | 3.74 | 1.63 | E | 6.99 | 3.05 |
| 4 | R | 3.37 | 1 | L | 3.25 | 0.96 | I | 3.17 | 0.94 | P | 2.27 | 0.67 | Q | 6.91 | 2.05 |
| 5 | I | 3.42 | 1 | V | 3.19 | 0.93 | T | 2.68 | 0.78 | H | 1.55 | 0.45 | E | 5.82 | 1.70 |
| 8 | I | 3.62 | 1 | P | 2.44 | 0.67 | G | 4.8 | 1.32 | E | 2.6 | 0.72 | R | 6.22 | 1.72 |
| Mean | 1 | 0.97 | 1.19 | 0.87 | 2.13 | ||||||||||
aa, amino acid present at that position.
Amount normalized against the standard PTH mixture, not corrected for the sequencing yield.
Relative amount of that amino acid normalized against the amino acid of HoxF at the same position.