TABLE 2.
Specific activities (nmol min−1 mg cell protein−1) of enzymes of the reductive and oxidative TCA cyclea
| Enzyme activity tested | Sp act (nmol min−1 mg protein−1)
|
|
|---|---|---|
| Thiomicrospira denitrificans | Candidatus Arcobacter sulfidicus | |
| Fumarate reductase | 220 | n.d. |
| Fumarate hydratase | 390 | n.d. |
| Isocitrate dehydrogenase | 1,100 | 2,300 |
| Malate dehydrogenase | ||
| NADPH | 830 | 810 |
| NADH | 960 | 510 |
| 2-Oxoglutarate dehydrogenase | ||
| NADP+ | <1 | <1 |
| NAD+ | <1 | <1 |
| Pyruvate dehydrogenase | ||
| NADP+ | <1 | <1 |
| NAD+ | <1 | <1 |
a
Mean values were obtained from three independent experiments. Standard errors were less than ±20%. The assays were carried out at 25°C. n.d., not determined.