TABLE 1.
Primers used in this study
| Primer | Sequencea | Product size (bp) |
|---|---|---|
| HtrA-SphI-FW | GGACCTCCCTCTGCATGCATAAAGGCTCGA | |
| HtrA-PstI-RV | GGAATCTTATTTCTGCAGGCTAATTTTGCTG | |
| PhtrA-SacI-FW2 | TGTAAATAGCGAGCTCCATTTTCTGA | |
| htrA-SphI-RV | GATTATGGTGCATGCAGACTTATTG | |
| Spo0J-flanking-FW | GGGTTCTAAGCGCCACATTA | |
| Spo0J-flanking-RV | TGAGAAAGTTTTCCACAGGTGA | |
| HtrAspo0J-FW | CGCTGACCCTCTTTTTCA | |
| HtrAspo0J-RV | TGGGCTGTTTGATGTGGAT | |
| For real-time PCR | ||
| gtfB-FW | AGCAATGCAGCCATCTACAAAT | 98 |
| gtfB-RV | ACGAACTTTGCCGTTATTGTCA | |
| gtfC-FW | CTCAACCAACCGCCACTGTT | 98 |
| gtfC-RV | GGTTTAACGTCAAAATTAGCTGTATTAGC | |
| gtfD-FW | CACAGGCAAAAGCTGAATTAACA | 83 |
| gtfD-RV | GAATGGCCGCTAAGTCAACAG | |
| ftf-FW | CAGCTAGTACACCGGAAGTAGG | 124 |
| ftf-RV | GCAATCTTACGAGCCTGTTCTG | |
| htrA2-FW | AAGTTGTTAGACCCGCTCTTG | 101 |
| htrA2-RV | ACCGCTTGTGACATCACTTGG | |
| spo0J-FW | AATCCCTATCAACCTCGACTGC | 142 |
| spo0J-RV | GCCTTTCTCCTGCAACCAAATC |
a
Italics indicate restriction endonuclease sites.