TABLE 2.
Real-time PCR-based expression files of gtfB, gtfC, gtfD, and ftf genes
| Gene | Relative quantity of mRNA (copies/μl)a
|
Ratiob | |
|---|---|---|---|
| UA159 | SAB2 | ||
| gtfB | 1.03E+05 ± 3.00E+04 | 4.70E+04 ± 1.00E+04 | 1:0.45 |
| gtfC | 3.47E+06 ± 9.00E+05 | 2.18E+06 ± 8.00E+05 | 1:0.63 |
| gtfD | 7.52E+05 ± 4.00E+05 | 1.19E+06 ± 9.00E+05 | N/D |
| ftf | 4.61E+05 ± 2.00E+05 | 7.52E+05 ± 4.00E+05 | N/D |
a
Following reverse transcription from 1 μg of total RNA from S. mutans strains UA159 and SAB2, the amounts of gtf and ftf gene cDNA were determined by real-time PCR using SYBR green. The data represent means±standard deviations which were obtained from three different RNA preparation and reverse-transcription reactions. See the text for experimental procedures in detail.
b
Ratio of UA159 mRNA to SAB2 mRNA. N/D indicates that there was statistically no difference (P < 0.01; Student's t test).