TABLE 3.
Induction of the htrA gene by CSP treatment over time by using real-time PCRa
| Parameter | Parameter by time after adding CSP (min)
|
||||
|---|---|---|---|---|---|
| 0 | 5 | 10 | 20 | 30 | |
| Relative quantity of htrA mRNA (× 105 copies/μl) | 2.03 ± 0.26 | 2.04 ± 0.82 | 2.99 ± 0.36 | 7.09 ± 0.44 | 4.05 ± 0.11 |
| n-fold induction | 1.0 | 1.5 | 3.5 | 2.0 | |
a
Early-exponential-phase culture of S. mutans UA159 was supplemented with CSP and then removed over time. Following reverse transcription from 1 μg of total RNA, the amount of htrA gene cDNA was determined by real-time PCR using SYBR green. These are typical results representative of two independent experiments. The data represent means ± standard deviations. See the text for more details.