FIG. 5.

Effects of a cloned metA gene on growth inhibition by acid. Cultures were inoculated by 1/1,000 dilution of a stationary phase minimal culture into 5 ml of NCE glucose medium containing 2.5 mM sodium benzoate and the indicated amount of IPTG. Ampicillin (0.1 mg/ml) was present for the strain carrying the pMetA plasmid. The averages for two independent cultures are plotted, and error bars represent the differences between the two determined values. OD₆₅₀, optical density at 650 nm.