TABLE 1.

ORFs with putative homologues in other phages or cellular organisms or identified as a virion structural protein by mass spectroscopy^a

ORF	Size (aa)	T4 similarity	E-value	Comment
1	295		2e-66	Possible Dam methylase
12	134		3e-25	Possible prohibitin.
16	136		5e-06	Similar to KVP40 CDS 229
53	170		4e-31	Similar to Gloeobacter violaceus hypothetical protein
60	200		4e-07	Similar to Trichodesmium hypothetical protein; contains a prolyl 4-hydroxylase domain
79	130	gp25	2e-09	Baseplate wedge subunit
80	602	gp6	3e-40	Baseplate wedge subunit
81	3048		3e-13	Similar to Novosphingobium aromaticivorans hypothetical protein
82	175			Virion structural protein
83	634	gp8	4e-15	Baseplate wedge subunit, virion structural protein
86	1251			Virion structural protein
87	168			Virion structural protein
88	3338		9e-5	Similar to phage Aeh1 hypothetical protein
89	306			Virion structural protein, contains a fibrinogen domain
90	327		9e-5	Virion structural protein, contains a pentraxin domain
91	379			Virion structural protein
93	267	gp13	2e-07	Neck protein, virion structural protein
94	292	gp14	4e-16	Neck protein
95	266	gp15	7e-26	Proximal tail sheath stabilizer, virion structural protein
96	139	gp16	3e-05	Terminase subunit
100	548	gp17	1e-97	Terminase large subunit
102	743	gp18	5e-62	Contractile tail sheath protein, virion structural protein
103	204	gp19	4e-17	Tail tube protein, virion structural protein
104	564	gp20	0	Portal vertex protein, virion structural protein
106	214	gp21	e-105	Prohead protease
107	392	gp22	e-112	Prohead core protein, virion structural protein
108	468	gp23	0	Major capsid protein, virion structural protein
110	169	gp3	0.073	Tail completion protein, virion structural protein
111	157	UvsY	0.039	Recombination and repair
112	487	UvsW	6e-61	DNA helicase
114	164	gp55	7e-13	Sigma factor for late transcription
117	349	gp47	1e-36	Recombination endonuclease
119	576	gp46	2e-75	Recombination protein
121	372		5e-26	Partial similarity to Salmonella enterica cobalt insertion protein CobS; has a MoxR-like ATPase domain
122	221	gp45	8e-11	Sliding clamp
123	315	gp44	2e-45	Sliding clamp loader subunit
126	59		6e-06	Similar to N-terminal portion of Nostoc methyl transferase
127	132		7e-13	Similar to C-terminal portion of Nostoc methyl transferase
129	128	gp62	1e-12	Sliding clamp loader subunit
130	142	RegA	2e-39	Translation repressor of early genes,
131	137		1e-19	Small heat shock protein (hsp 20 family),
134	830	gp43	1e-136	DNA polymerase
135	343	UvsX	3e-35	RecA-like recombination protein
136	470	gp41	3e-90	DNA primase-helicase subunit,
137	135		5e-10	Similar to conserved hypothetical bacterial protein family; contains a putative nucleotide pyrophosphohydrolase domain
146	316			Virion structural protein
150	329	gp61	1e-33	DNA primase subunit
151	776	NrdA	1e-158	Ribonucleotide reductase alpha subunit
152	391	NrdB	1e-108	Ribonucleotide reductase beta subunit
155	136		1e-39	Similar to Nostoc lysin; contains a phage lysozyme domain
159	120		1e-20	Possesses a rare lipoprotein A-2 domain,
163	81		7e-06	Glutaredoxin
167	75		1e-08	Similar to N-terminal portion of putative tryptophan halogenase; contains a tryptophan halogenase domain
168	410		1e-08	Similar to C-terminal portion of tryptophan halogenase; contains a tryptophan halogenase domain
169	279		33-13	Possesses an S-layer homology domain
171	162		5e-04	High-light inducible protein
173	39		1e-4	High-light inducible protein
174	1095			Virion structural protein, possible adhesin
175	1058		2e-07	Similar to putative Leptospira interrogans outer membrane protein
176	1177			Virion structural protein, possible adhesin
177	359		9e-65	Photosynthetic reaction centre protein D1, contains a 212 bp intron
178	143		3e-67	Similar to bacteriophage phiYeO3-12 ORF; contains an endonuclease VII domain
180	353		2e-58	Photosynthetic reaction centre protein D2
182	83		7e-04	Partial similarity to glutaredoxin
185	78		1e-19	Similar to a conserved family of cyanobacterial proteins
186	287	Rnh	9e-49	RNase H
190	110		1e-23	S-adenosylmethionine decarboxylase
194	213		2e-61	Thymidylate synthase-complementing protein (homologue in phage P60)
196	250		3e-21	Partial similarity to PhoH, ATPase related to phosphate starvation (homologue in phage P60).
197	229		7e-14	Similar to hypothetical Nostoc protein; contains a RecB domain
198	249	gp33	0.003	gp33 phage 44RR
200	295	gp32	5e-52	Single-strand DNA binding protein
201	220	gp53	1e-08	Base plate wedge component.
202	332	gp48	2e-08	Base plate, tail tube associated; virion structural protein
203	220	gp2	8e-12	DNA end protector protein
204	145	gp4	8e-33	Head completion protein.
206	238	gp26	3e-19	Base plate hub subunit
207	57		6e-05	Possible base plate hub assembly catalyst
208	1167		9e-10	Partial similarity to bacteriophage CP-1 orf18
209	749			Contains a domain found in membrane proteins related to metalloendopeptidases
211	981	gp5?	4e-08	Partial similarity baseplate hub subunit and tail lysozyme
212	367	gp5?	3e-04	Partial similarity baseplate hub subunit and tail lysozyme
213	114		4e-11	Similar to marine Synechococcus and Prochlorococcus conserved hypothetical proteins
216	175		3e-20	Similar to cyanobacterial CpeT
218	193		2e-06	Partially similar to prolyl 4-hydroxylase alpha subunit
221	295			Virion structural protein
222	567		1e-15	Partially similar to Methanosarcina hypothetical protein; shares a similar C-terminal domain with ORFs 223 and 224
223	560		5e-07	Partially similar to Methanosarcina hypothetical protein; shares a similar C-terminal domain with ORFs 222 and 224; virion structural protein
224	582		3e-11	Partially similar to Methanosarcina hypothetical protein; shares a similar C-terminal domain with ORFs 222 and 223
225	1037		5e-07	Possible short tail fiber; virion structural protein
226	238		2e-16	Similar to a conserved bacterial family of proteins; contains a prolyl 4-hydroxylase domain
228	231		2e-21	Similar to a conserved bacterial family of proteins; contains a prolyl 4-hydroxylase domain
229	169			Contains a prolyl 4-hydroxylase domain
233	415	UvsW	2e-04	DNA or RNA helicase.
235	202		9e-13	Similar to Ralstonia hypothetical protein
239	186		5e-15	Similar to Helicobacter hypothetical protein

^aMass spectroscopy data are from our laboratory (unpublished results).