TABLE 2.
Interactions between PspA fragments and PspF using an in vivo bacterial two-hybrid system in E. coli
| Fusiona
|
% repressionb (mean ± SD) | |
|---|---|---|
| pRS658 | pSR659 | |
| pspA | pspF | 69 ± 1 |
| pspF | pspA | 73 ± 1 |
| pspA1-186 | pspF | 65 ± 6 |
| pspA1-110 | pspF | 26 ± 3 |
| pspA110-222 | pspF | 0 ± 6 |
| pspA67-222 | pspF | 19 ± 9 |
| pspA67-186 | pspF | 2 ± 5 |
| pspA | pspFW56A | 38 ± 0.8 |
a
Gene fused to either the wild-type lexA DBD (pSR658) or the mutant lexA DBD (pSR659).
b
Percent repression of β-galactosidase activity relative to strain SU202 with pSR658 and pSR659 alone. The average activity of the vector control was 4,689 ± 246 Miller units (nonrepressed activity).