Fig. 7.

Effect of FABP7 overexpression in primary mouse hippocampal astrocytes. A Relative luminescence produced by firefly luciferase expressed under an NF-κB-driven promoter 48 h after GFP, FABP7, or FABP7(3A) overexpression in hippocampal astrocytes isolated from non-transgenic mice. Confluent cultures were co-transduced with an NF-κB-driven firefly luciferase construct and a Renilla luciferase construct under the control of a constitutive promoter. 72 h later, cultures were transduced with adenovirus expressing GFP, wild-type FABP7, or FABP7(3A). Relative firefly luciferase luminescence was corrected by the amount of Renilla luciferase activity and expressed as a percentage of GFP control cells. B Real-time PCR analysis of Cxcl10, Nos2, Il6, Ptgs2, and Ccl5 mRNA expression 48 h after GFP, FABP7, or FABP7(3A) overexpression in primary hippocampal astrocyte cultures. mRNA levels were corrected by Rplp0 mRNA levels and expressed as a percentage of GFP control cells. For all panels, data are expressed as mean ± SD (*p < 0.05)