FIG. 4.

Biofilm formation by serovar Typhimurium strain BJ2710 or serovar Typhimurium BJ3402 (BJ2710 ΔwcaM) on chicken intestinal epithelium. (A and B) z-sections generated by confocal microscopy of BJ2710 and BJ3402, respectively, that were able to adhere to and grow as biofilm on the chicken intestinal epithelial surface. The organisms were originally labeled with GFP and appear white in the image. Panels C and D correspond to BJ2710 and BJ3402, respectively, and show a dramatic difference in the height and extent of BJ2710 biofilm which is 50 to 60 μm in height and covers the entire epithelial surface and BJ3402, which has adherent patches of organisms and occasional spikes of growth that are up to 10 μm in height.