TABLE 1.

Bacterial strains and plasmids used in the study

Strain or plasmid	Genotype or phenotypea	Source or reference
Strains
Escherichia coli DH12S	mcrA Δ(mrr-hsdRMS-mcrBC) F′ lacIq ΔM15	Invitrogen
Salmonella enterica serovar Typhimurium
SL1344	Virulent wild-type strain	36
LB5010	Serovar Typhimurium LT2 strain containing a complete fim gene cluster	36
BJ2508	BJ2710 derivative with a fimH::kan insertion; Kanr	3
BJ2710	SL1344 derivative containing an adhesive fimH gene	This work
BJ3402	BJ2710 derivative with a deletion of the wcaM gene	This work
BJ3458	BJ2710 derivative with a disruption of the hns gene	This work
BJ3512	BJ2710 derivative with a disruption of the yhjN gene	This work
BJ3537	BJ2710 derivative with a disruption of wcaA	This work
BJ3538	BJ2710 derivative with a disruption of wza	This work
BJ3468	BJ2710 derivative with a disruption of wcaM and hns	This work
Plasmids
pBDJ254	pGEM-T carrying the hns gene; Ampr	This work
pKD3	pANTSγ vector containing the cam template gene cloned from pSC140; Ampr	10
pKD4	pANTSγ vector containing the kan template gene cloned from pCP15; Ampr	10
pKD46	Temperature-sensitive red helper plasmid expressing araC-ParaB and γβexo from λ phage; Ampr	10
pMRP9-1	GFP-expressing plasmid; Ampr	E. P. Greenberg
pNAL100	pGEM-T vector carrying the wcaM gene; Ampr	This work
pISF204	pBR322 vector containing fimH and fimF; Ampr	17
pBBRMCS-1	Plasmid encoding GFP; Camr	22

^aAmp^r, ampicillin resistant; Kan^r, kanamycin resistant; Cam^r, chloramphenicol resistant.