FIG. 2.
Accumulation of the σX protein requires ComW. The WT (CP1250) and a comW mutant (CP1376), a C-His-tagged ComW mutant (CP1802; 0- to 25-min samples in panel C), and an N-His-tagged ComW mutant (CP1805; 0- to 25-min samples in panel D) were treated with CSP (at time zero), and samples were collected as described in Materials and Methods at the indicated times (in minutes). An extract representing 1 ml of culture was loaded into each well. Samples of CP1376 from two independent preparations were loaded onto different gels (A and B). The gels were assayed by Western blotting using an antibody against σX.