FIG. 4.
Deletion of both comW and clpP leads to the accumulation of σX after competence induction. The wild-type (CP1250; 0- to 60-min samples in panel A), a comX mutant (CPM8), a clpP single mutant (CP1359; 0- to -80-min samples in panel B), and a comW clpP double mutant (CP1815; 0- to 80-min samples in panel C) were induced by CSP (at time zero), and samples were withdrawn at the indicated times (in min) after induction. The indicated amount of each extract (0.1 ml of original culture per μl extract) were assayed by Western blotting analysis and probed with anti-ComX antibody.