TABLE 1.
Pneumococcal strains used in this study
| Strain | Description | Sourcea |
|---|---|---|
| CP1250 | S. pneumoniae Rx derivative; low β-galactosidase activity; hex malM511 str-1 bgl-1 Hex− Mal- Smr Bga− | 25 |
| CP1500 | hex nov-r1 bry-r str-1 ery-r1 ery-r2 Novr | 5 |
| CP1359 | CP1250, but clpP::tet Tetr | 17 |
| CP1376 | CP1250, but comW::KANT Kanr | 27 |
| CP1548 | CP1250, but cglA::pEVP3 Cmr | 16 |
| CP1601 | CP1250, but celB::pEVP3 Cmr | 16 |
| CP1714 | comW::KANT ssbB::pEVP3::ssbB+ Cmr Kanr | CP1376 × CPM7 |
| CP1718 | cglA::pEVP3 Cmr Kanr | CP1376 × CP1548 |
| CP1719 | celB::pEVP3 Cmr Kanr | CP1376 × CP1601 |
| CP1721 | CP1250, but comW::pEVP3::comW+ Cmr | pCKS03 × CP1250 |
| CP1723 | comW::KANT comX1::erm comX2::Tet Ermr Kanr Tetr | CP1376 × CPM8 |
| CP1724 | comW::KANT comX1::pEVP3::comX1+ Cmr Kanr | CP1376 × CPM3 |
| CP1731 | CP1721, but comE::erm Ermr Cmr | This study |
| CP1802 | CP1250, but C terminus-V5-H6-ComW-pEVP3 Cmr | pCKS07 × CP1250 |
| CP1805 | CP1250, but N terminus-H6-Xa-ComW-KANT Kanr | This study |
| CP1815 | clpP::tet comW::KANT Kanr Tetr | CP1376 × CP1359 |
| CP1816 | clpP::tet N-H6-Xa-ComW-KANT Kanr Tetr | CP1359 × CP1805 |
| CP1820 | comW::KANT clpP::tet cglA::pEVP3 Cmr Kanr Tetr | CP1376 × CP1821 |
| CP1821 | clpP::tet cglA::pEVP3 Cmr Tetr | CP1548 × CP1359 |
| CP1822 | comW::KANT clpP::tet celB::pEVP3 Cmr Kanr Tetr | CP1376 × CP1823 |
| CP1823 | clpP::Tet celB::pEVP3 Cmr Tetr | CP1601 × CP1359 |
| CP1850 | clpP::Tet C-V5-H6-ComW-pEVP3 Cmr Tetr | CP1359 × CP1802 |
| CP1851 | CP1250, but clpE::erm Ermr | This study |
| CP1852 | comW::KANT clpE::erm clpP::tet Ermr Kanr Tetr | CP1376 × CP1853 |
| CP1853 | clpE::erm clpP::tet Ermr Tetr | CP1359 × CP1851 |
| CP1854 | comW::KANT clpE::Erm Ermr Kanr | CP1376 × CP1851 |
| CPM3 | CP1250, but comX1::pEVP3::comX1+ Cmr | 16 |
| CPM4 | CP1250, but comX1::Erm comX2::pEVP3 Cmr Ermr | 16 |
| CPM7 | CP1250, but ssbB::pEVP3::ssbB+ Cmr | 16 |
| CPM8 | CP1250, but comX1::Erm comX2::tet Ermr Tetr | 16 |
a
Construction of a strain by transformation between two different mutant strains is shown as DNA × recipient.