TABLE 3.
CSP induction of lacZ fusions to comW, ssbB, celB, and cglA genes in different genetic backgrounds
| Strain and relevant genotype | lacZ fusion sitea | Beta-galactosidase activityb
|
|
|---|---|---|---|
| W/o CSP | CSP | ||
| CP1721 WT | comW | 0.5 ± 1 | 140 ± 100 |
| CP1731 comE | comW | 1 ± 1 | 0.5 ± 1 |
| CP1723 comX | comW | 0.5 ± 1 | 450 ± 250 |
| CPM7 WT | ssbB | 0 | 80 ± 40 |
| CP1714 comW | ssbB | 0.5 ± 1 | 10 ± 1 |
| CP1601 celB | celB | 1 ± 1 | 60 ± 40 |
| CP1719 celB comW | celB | 2 ± 2 | 2 ± 1 |
| CP1822 celB comW clpP | celB | 1 ± 1 | 5 ± 5 |
| CP1823 celB clpP | celB | 2 ± 1 | 30 ± 20 |
| CP1548 cglA | cglA | 0 | 300 ± 300 |
| CP1718 cglA comW | cglA | 1 ± 1 | 20 ± 10 |
| CP1820 cglA comW clpP | cglA | 1 ± 1 | 20 ± 20 |
| CP1821 cglA clpP | cglA | 2 ± 0 | 150 ± 100 |
a
lacZ reporter gene was inserted into indicated locus.
b
In each of three experiments, cells were grown in complete CAT medium supplemented with 8 mM HCl to an OD550 of 0.05, and a noncompetent culture and a parallel sample of the same culture treated with CSP for 20 min were prepared. Both samples were lysed and assayed for β-galactosidase as described in Materials and Methods. The average activity (Miller units) for three experiments is also shown, with standard deviations (SD) or range. W/o, without.