TABLE 4.
Quantification of ComX in various pneumococcal strains
| Protein sample and timea | IDVb | Avg relative amt (%) ± rangec |
|---|---|---|
| CP1250 (WT) 15 | 100 | |
| CP1376 (ΔcomW) 15 | 600 ± 180 | 8 ± 1 |
| CP1802 (C-his-ComW) 15 | 800 ± 200 | 11 ± 0.1 |
| CP1805 (N-his-ComW) 15 | 750 ± 400 | 9 ± 4 |
| CP1815 (ΔclpP, ΔcomW) 80 | 100 | |
| CP1815 (ΔclpP, ΔcomW) 15 | 1,300 ± 170 | 27 ± 2 |
| CP1359 (ΔclpP) 15 | 750 ± 100 | 13 ± 2 |
| CP1359 (ΔclpP) 80 | 1,000 ± 100 | 18 ± 0.5 |
| CP1250 (WT) 15 | 550 ± 50 | 8 ± 4 |
a
Samples were prepared at indicated times (in min) post-CSP treatment.
b
The intensity of each band was converted into an IDV using Alpha-Ease FC.
c
The average of the relative amounts of ComX determined by two independent experiments is shown, with a range. The relative amount of the ComX protein was determined by the IDV of each sample and standard curve graphs. (The graph of serially diluted samples of CP1250 at 15 min [Fig 3A] was used for CP1376, CP1802, and CP1805; the graph of serially diluted samples of CP1815 at 80 min [Fig 3B] was used for CP1815 at 15 min, CP1359 at 15 min, CP1359 at 80 min, and CP1250 at 15 min.)