TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source and/or reference |
|---|---|---|
| Strains | ||
| E. coli XL1-Blue | Host strain used for cloning | Stratagene |
| M. catarrhalis | ||
| 7169 | Wild-type strain, pediatric middle ear isolate | Howard Faden (Children's Hospital, Buffalo, N.Y.); 34, 35 |
| 7169::lgt1KB | lgt1 kanamycin-resistant isogenic mutant of strain 7169 | This study |
| 7169::lgt2KM | lgt2 kanamycin-resistant isogenic mutant of strain 7169 | This study |
| 7169::lgt3KH | lgt3 kanamycin-resistant isogenic mutant of strain 7169 | This study |
| 7169::lgt1KB(REV) | 7169::lgt1KB mutant complemented to create a wild-type lgt1 revertant | This study |
| 7169::lgt1KM(REV) | 7169::lgt2KM mutant complemented to create a wild-type lgt2 revertant | This study |
| 7169::lgt3KH(REV) | 7169::lgt3KH mutant complemented to create a wild-type lgt3 revertant | This study |
| H. ducreyi | ||
| 35000 | Wild-type strain isolated in Winnipeg, Canada, binds MAb 1B2-1B7 | 17 |
| 35000glu− | Isogenic LOS mutant reported previously to be derived from strain 35000 by disruption of lgtF gene and insertion of a cat cassette, does not bind MAb 1B2-1B7 | 11 |
| 35000glu−(pLS3PR-E) | Strain derived from the electroporation of pLS3PR-E into strain 35000glu− to complement the inactivated lgtF gene, binds MAb 1B2-1B7 | This study |
| 35000glu−(pLS3IN-1) | Strain derived from the electroporation of pLS3IN-1 into strain 35000glu− to complement the inactivated lgtF genes, does not bind MAb 1B2-1B7 | This study |
| Plasmids | ||
| pGEM-T easy | Commercially available vector used for TA cloning and mutagenesis | Promega |
| pUC18K | Construct containing aphA-3 kanamycin resistance cassette | 37 |
| pLGT-1KE | lgt1 TA cloned into pGEM-T easy | This study |
| pLGT-2KE | lgt2 TA cloned into pGEM-T easy | This study |
| pLGT-3KE | lgt3 TA cloned into pGEM-T easy | This study |
| pLGT-1kanB | lgt1 containing an internal deletion and insertion of aphA-3 cassette | This study |
| pLGT-2kanM | lgt2 containing an internal deletion and insertion of aphA-3 cassette | This study |
| pLGT-3kanH | lgt3 containing an internal deletion and insertion of aphA-3 cassette | This study |
| pLS88 | E. coli-H. ducreyi shuttle vector, Kanr, Smr, Sulr | 6 |
| pLS3PR-E | pLS88 vector with a 2.2-kb insert of M. catarrhalis 7169 lgt3 gene (includes 265 bp of 5′ flanking DNA) | This study |
| pLS3IN-1 | pLS88 vector with a 1.3-kb insert of M. catarrhalis 7169 lgt3 gene (internal portion) | This study |