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. 2023 Apr 19;20(1):83–89. doi: 10.1007/s11302-023-09941-2

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Fig. 3 — ATP in fetal bovine serum (FBS) is biologically available. A NADH absorbance increase at two different FBS concentrations. B NADH absorbance increase in FBS sample treated with apyrase (Apy) or inactivated apyrase. C [Ca²⁺]_i levels were measured with Fura 2/AM in HEK293-P2X7R cells (2 × 10.⁶) incubated overnight in DMEM-F12 medium supplemented with FBS from different commercial sources (Gibco, Euroclone, and Sigma), rinsed, resuspended in saline solution (see the “Materials and methods” section), and stimulated with ATP (1mM)