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Effects of DPD/AI-2 on B. burgdorferi VlsE protein expression. Bacteria were cultured in the presence of indicated concentrations of in vitro-synthesized DPD/AI-2 and then analyzed by immunoblotting using VlsE-directed antiserum. As controls for equal loading, membranes were also analyzed using a monoclonal antibody directed against the constitutively expressed FlaB (flagellin) protein.
