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. 2024 Jan 30;15:889. doi: 10.1038/s41467-024-45075-8

Fig. 5. Amphotericin B partially relieves a block to WA1 Spike- and Delta Spike-mediated infection in primary nasal epithelia.

Fig. 5

A Primary human nasal epithelial cells (pooled from 14 donors) were cultured at the air–liquid interface. IFITM2/3 levels were determined by anti-IFITM2/3 immunofluorescence, and DAPI was used to stain nuclei. A 3D reconstruction (xyz) of stacked confocal images is shown. B Primary human nasal epithelial cells (pooled from 14 donors) were cultured at the air–liquid interface, pre-treated with 1 µM Amphotericin B for 2 h or untreated, and inoculated with 10,000 plaque-forming units of WA1 (WA1 Spike), WA1 (Delta Spike), or WA1 (BA.1 Spike). mCherry fluorescence was measured in fixed tissue at 24 h post-inoculation by high-content imaging of the entire well. C Quantification of mCherry fluorescence intensity from two independent experiments is shown. Scale bar = 300 µm. Ampho B; amphotericin B. Source data are provided as a Source Data file.