FIG. 4.

Metabolic flux distribution in the Crp mutant (bottom values) and the parent strain (top values) (A) and in the Cya mutant (bottom values) and the parent strain (top values) (B) during exponential aerobic growth on glucose. For clarity only the lower part of metabolism is shown. Net molar fluxes (expressed in millimoles per gram per hour) were determined by ¹³C-constrained flux analysis from two separate experiments with 100% [1-¹³C]glucose and with a mixture of 20% [U-¹³C]glucose and 80% unlabeled glucose. In all cases the standard deviation was less than 0.2 mmol g⁻¹ h⁻¹. Fluxes to biomass building blocks and extracellular products are enclosed in boxes.