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. 2023 Dec 23;300(2):105594. doi: 10.1016/j.jbc.2023.105594

Figure 3.

Figure 3

Coimmunoprecipitation of ABCB5β-ABCB6 and ABCB5β-ABCB9 demonstrates the presence of these heterodimers in Mel JuSo and UACC-257 cells. The Mel JuSo (A) or UACC-257 (B) proteins were immunoprecipitated (IP) with either an anti-ABCB5, anti-ABCB6, or anti-ABCB9 antibody. The precipitated proteins were revealed by Western blotting after SDS-PAGE using the corresponding antibody indicated on the right side of each blot. Fifteen micrograms of total proteins from the starting cell lysate were loaded in the first lane, while the total IP eluate was loaded on the gel. ABCB5β was expressed in High-Five insect cells, and total membrane proteins were prepared and loaded on the gel (High5 ABCB5β) as a complementary molecular weight marker when using anti-ABCB5. An isotype control was performed to determine the specificity of the signal obtained in Western blot.