TABLE 4.
Urease-specific serum and gastric IgG subclass responses
| Route of immunizationa | Antiurease IgG1/IgG2a antibody ratio in indicated fluid
|
|||
|---|---|---|---|---|
| Prechallenge with H. pylori
|
Postchallenge with H. pylori
|
|||
| Serum | Gastric juice | Serum | Gastric juice | |
| p.o. | 0.16 | 0.35 | 0.25 | 0.19 |
| r. | 2.51 | 1.65 | 0.88 | 0.49 |
| i.n. | 0.29 | 0.18 | 0.08 | 0.09 |
a
Weekly immunizations with rUre plus LT were given on days 0, 7, 14, and 21 by either the p.o. (50 μl of soluble antigen delivered by pipette into the buccal cavity), the i.n. (5 μl of soluble antigen delivered to each external naris of unanesthetized mice), or the rectal (r.) (25 μl of soluble antigen delivered with an olive-tipped catheter directly into the rectum) route.