Fig. 3. (a) FeND interaction with A549 cells imaged using laser confocal fluorescence images captured through a stepwise Z-scan at intervals of 1.5 μm. For LSCM, cell cytoplasm was stained with Hoechst and the cell cytoplasm was stained with DIOC5, respectively. Two-photon fluorescence lifetime image of (b) A549 cells and (c) A549 cells incubated with FeND. (c) Fluorescence lifetime decays for FeND fluorescence and A549 cell autofluorescence using two-photon excitation.
