FIG. 5.

Reactivity of MAb 64/32 with infected erythrocytes, normal bovine serum, and purified IgM. B. bigemina-infected erythrocytes (lanes 1, 5, and 9), normal bovine serum (lanes 2, 6, and 10), purified bovine IgM (lanes 3, 7, and 11), or purified bovine IgG (lanes 4, 8, and 12) were separated by SDS-PAGE under reducing conditions. The immunoblotted proteins were probed with MAb 64/32 (lanes 1 to 4), MAb BIg73 (anti-bovine μ heavy chain) (lanes 5 to 8), or isotype control MAb Tryp1E1 (lanes 9 to 12). The 87-kDa molecular size standard is indicated on the left.