Table 2.
Antimicrobial susceptibility test data for 49 strains of RGM from cats with mycobacterial panniculitis determined using a disc diffusion method
| Strain | Identity | TMP5 | DO30 | GM10 | CIP5 | CLR15 | NOR10 | ENR5 | PB300 |
|---|---|---|---|---|---|---|---|---|---|
| (a) Species identification confirmed at a Mycobacteria Reference Laboratory | |||||||||
| 1 | M fortuitum(3) | R | R | S | NT | NT | S | NT | S |
| 2 | M fortuitum(3) | NT | NT | NT | NT | NT | NT | NT | NT |
| 3 | M fortuitum(f) | R | S | R | NT | NT | S | NT | S |
| 4 | M fortuitum(f) | R | S | R | NT | NT | S | NT | S |
| 5 | M fortuitum(f) | R | S | S | NT | NT | S | NT | S |
| 6 | M fortuitum(f) | R | R | S | NT | NT | S | NT | S |
| 7 | M fortuitum(f) | R | S | S | S | NT | S | NT | S |
| 8 | M fortuitum(f) | NT | NT | NT | NT | NT | NT | NT | NT |
| 9 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 10 | M smegmatis ‡ | S | S | S | R | NT | R | NT | S |
| 11 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 12 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 13 | M smegmatis | S | S | S | S | NT | S | NT | NT |
| 14 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 15 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 16 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 17 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 18 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 19 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 20 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 21 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 22 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 23 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 24 | M smegmatis | S | NT | S | NT | NT | S | NT | S |
| 25 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 26 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 27 | M smegmatis | S | S | S | S | NT | S | S | S |
| (b) Species identification based on susceptibility to trimethoprim | |||||||||
| 28 | M fortuitum | R | R | S | S | I | NT | S | NT |
| 29 | M smegmatis | S | S | S | NT | NT | S | NT | S |
| 30 | M smegmatis † | S | NT | S | NT | NT | S | NT | S |
| 31 | M smegmatis † | S | S | S | NT | NT | S | NT | S |
| 32 | M smegmatis | S | S | S | S | NT | S | NT | NT |
| 33 | M smegmatis | S | S | S | S | NT | NT | NT | S |
| 34 | M smegmatis | S | S | S | S | NT | NT | NT | S |
| 35 | M smegmatis | S | S | S | S | NT | NT | NT | S |
| 36 | M smegmatis | S | S | S | S | NT | NT | NT | S |
| 37 | M smegmatis | S | S | S | S | NT | NT | NT | S |
| 38 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 39 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 40 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 41 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 42 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 43 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 44 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 45 | M smegmatis | S | S | S | S | NT | S | NT | S |
| 46 | M smegmatis | S | S | S | S | NT | NT | S | NT |
| 47 | M smegmatis | S | S | S | S | NT | S | S | NT |
| 48 | M smegmatis | S | S | S | S | NT | S | S | S |
| 49 | M smegmatis ‡ | S | S | S | R | S | R | R | NT |
| Inhibition zone (mm)* | 20 | 30 | 16 | 30 | 30 | 17 | 30 | 10 | |
Organisms conformed to growth and microscopic characteristics of rapidly growing mycobacterial species and were confirmed as M smegmatis by testing at the Mycobacteria Reference Laboratory.
Isolated following prolonged monotherapy with enrofloxacin or ciprofloxacin.
DO30, doxycycline 30 μg; TMP5, trimethoprim 5 μg; GM10, gentamicin 10 μg; NOR10, norfloxacin 10 μg; CIP5, ciprofloxacin 5 μg; ENR5, enrofloxacin 5 μg; CLR15, clarithromycin 15 μg; PB300, polymyxin B 300 μg. S, susceptible; R, resistant; I, intermediate; NT, not tested.
M fortuitum(3), unnamed third biovar of M fortuitum; M fortuitum(f), M fortuitum biovar fortuitum.
Zone diameter to be exceeded to be classed as susceptible.