Figure 9. Glucocorticoid induces expression of a pattern-recognition receptor.

(A) THP-1^control and THP-1^GR-KD macrophages were left untreated or pre-treated with dexamethasone (1 μM) for 24 hours, followed by immunoblotting of the whole-cell lysates for TLR2 (top). The same membrane was stripped and immunblotted for β-actin (bottom). Data shown in representative of three independent experiments. (B) Relative densitometric measurement of TLR2 protein normalized for β-actin. Data shown are the mean density (arbitrary units) of three independent experiments. *p<0.05, **p<0.01, ***p<0.001. p values for the THP-1^GR-KD cells are in comparison to their corresponding THP-1^control cells.