FIG. 2.

IFN-γ mRNA accumulation was determined in skeletal muscle (A) and spleens (B) from TNFRp55^−/− and WT mice at 0 or 30 days after infection with T. cruzi (CA-I). Total RNA was obtained from spleens or skeletal muscle of individual mice, and IFN-γ and β-actin mRNA were measured in a competitive PCR assay. The mean moles of IFN-γ mRNA per mole of β-actin mRNA (used as a housekeeping gene) from four cDNA samples per group, of one of two independent experiments, are depicted. ∗, significantly different from the value for noninfected WT or mutant controls (P < 0.001, Mann-Whitney-U Wilcoxon test). #, significantly different from the value for WT infected mice (P < 0.05, Mann-Whitney-U Wilcoxon test).